Protein Concentration:Article Title: Chronic maternal exposure to low-dose PM 2.5 impacts cognitive outcomes in a sex-dependent manner.
Article Snippet: .. Protein concentration was measured by DC protein assay, and samples were separated in the CriterionTMTGX Stain-Free Precast Gel (Bio-Rad, USA) and then transferred onto PVDF membranes (Bio-Rad) followed by blocking with 2 % BSA. .. Membranes were incubated with primary antibodies (OXPHOS complexes [1:2500, Abcam, Cambridge, UK], Drp-1 [1:2000, Novus Biotechnology, Colorado, USA], Opa-1 [1:2000, Novus Biotechnology, Littleton, USA], MnSOD [1:2000, Millipore, MA, USA], cytochrome c oxidase subunit [Cox] IV [1:10000, Novus Biological], β-actin [1:3000, Santa Cruz Biotechnology, Texas, USA]) overnight at 4 ◦C and incubated with secondary antibodies (goat anti-rabbit or rabbit anti-mouse IgG horseradish peroxidase-conjugated secondary antibodies, 1:5000 for OPA-1, MnSOD, OXPHOS complexes; 1:2000 for Drp-1; 1:1000 for β-actin, Santa Cruz Biotechnology; 1:10000 for CoxIV, Bio-Rad) for 1 h at room temperature.
DC Protein Assay:Article Title: Chronic maternal exposure to low-dose PM 2.5 impacts cognitive outcomes in a sex-dependent manner.
Article Snippet: .. Protein concentration was measured by DC protein assay, and samples were separated in the CriterionTMTGX Stain-Free Precast Gel (Bio-Rad, USA) and then transferred onto PVDF membranes (Bio-Rad) followed by blocking with 2 % BSA. .. Membranes were incubated with primary antibodies (OXPHOS complexes [1:2500, Abcam, Cambridge, UK], Drp-1 [1:2000, Novus Biotechnology, Colorado, USA], Opa-1 [1:2000, Novus Biotechnology, Littleton, USA], MnSOD [1:2000, Millipore, MA, USA], cytochrome c oxidase subunit [Cox] IV [1:10000, Novus Biological], β-actin [1:3000, Santa Cruz Biotechnology, Texas, USA]) overnight at 4 ◦C and incubated with secondary antibodies (goat anti-rabbit or rabbit anti-mouse IgG horseradish peroxidase-conjugated secondary antibodies, 1:5000 for OPA-1, MnSOD, OXPHOS complexes; 1:2000 for Drp-1; 1:1000 for β-actin, Santa Cruz Biotechnology; 1:10000 for CoxIV, Bio-Rad) for 1 h at room temperature.
Staining:Article Title: Chronic maternal exposure to low-dose PM 2.5 impacts cognitive outcomes in a sex-dependent manner.
Article Snippet: .. Protein concentration was measured by DC protein assay, and samples were separated in the CriterionTMTGX Stain-Free Precast Gel (Bio-Rad, USA) and then transferred onto PVDF membranes (Bio-Rad) followed by blocking with 2 % BSA. .. Membranes were incubated with primary antibodies (OXPHOS complexes [1:2500, Abcam, Cambridge, UK], Drp-1 [1:2000, Novus Biotechnology, Colorado, USA], Opa-1 [1:2000, Novus Biotechnology, Littleton, USA], MnSOD [1:2000, Millipore, MA, USA], cytochrome c oxidase subunit [Cox] IV [1:10000, Novus Biological], β-actin [1:3000, Santa Cruz Biotechnology, Texas, USA]) overnight at 4 ◦C and incubated with secondary antibodies (goat anti-rabbit or rabbit anti-mouse IgG horseradish peroxidase-conjugated secondary antibodies, 1:5000 for OPA-1, MnSOD, OXPHOS complexes; 1:2000 for Drp-1; 1:1000 for β-actin, Santa Cruz Biotechnology; 1:10000 for CoxIV, Bio-Rad) for 1 h at room temperature.
Blocking Assay:Article Title: Chronic maternal exposure to low-dose PM 2.5 impacts cognitive outcomes in a sex-dependent manner.
Article Snippet: .. Protein concentration was measured by DC protein assay, and samples were separated in the CriterionTMTGX Stain-Free Precast Gel (Bio-Rad, USA) and then transferred onto PVDF membranes (Bio-Rad) followed by blocking with 2 % BSA. .. Membranes were incubated with primary antibodies (OXPHOS complexes [1:2500, Abcam, Cambridge, UK], Drp-1 [1:2000, Novus Biotechnology, Colorado, USA], Opa-1 [1:2000, Novus Biotechnology, Littleton, USA], MnSOD [1:2000, Millipore, MA, USA], cytochrome c oxidase subunit [Cox] IV [1:10000, Novus Biological], β-actin [1:3000, Santa Cruz Biotechnology, Texas, USA]) overnight at 4 ◦C and incubated with secondary antibodies (goat anti-rabbit or rabbit anti-mouse IgG horseradish peroxidase-conjugated secondary antibodies, 1:5000 for OPA-1, MnSOD, OXPHOS complexes; 1:2000 for Drp-1; 1:1000 for β-actin, Santa Cruz Biotechnology; 1:10000 for CoxIV, Bio-Rad) for 1 h at room temperature.
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